Role of Catalase in Protection of Cancer Cells from Oxidative Stress induced by Binary Catalytic System “Teraphtal + Ascorbic acid”

The efficacy of a novel anti-tumor agent binaric catalitic system “teraphtal + ascorbic acid” [BCS (T+A)], generating reactive oxygen species, may depend on the activity of enzymes of the cellular antioxidant defense system including catalase (CAT). To evaluate the role of CAT in cancer cell defense from oxidative stress induced by BCS (T+A), we studied the following biomarkers: the expression level and basal activity of CAT in cells, and its sensitivity to specific inhibitor, aminotriazole (3-AT). We found that functionally active CAT was expressed constitutively in cultures of human tumor cells of different hystogenesis grown in vitro; at that the basal levels of CAT-protein expression and CAT-enzyme activity depend on cell types. The efficacy of CAT inhibiting by 3-AT (the parameter IC₅₀ 3-АТ) is the same for cells of all tested cultures, it ranges from 20 to 25 mM and does not depend on the level of CAT protein expression in cells. No direct correlation was found between the biological CAT characteristics and their sensitivity to BCS (T+A) for cells of different hystogenesis. In case of pharmacological CAT inhibition, the cytotoxic activity of BCS (T+A) is doubled, whereas the human tumor cell sensitization degree (increased sensitivity) to BCS (T+A) depends on their type.

• Т.А. Sidorova N.N. Blokhin Russian Cancer Research Center of RAMS, Moscow, Russian Federation ; ФГБУ «РОНЦ им. Н.Н. Блохина» РАМН, Москва, Российская Федерация
• M.S. Vagida N.N. Blokhin Russian Cancer Research Center of RAMS, Moscow, Russian Federation ; ФГБУ «РОНЦ им. Н.Н. Блохина» РАМН, Москва, Российская Федерация
• O.L. Kaliya State Scientific Center NIOPIC, Moscow, Russian Federation ; ФГУП Государственный научный центр «НИОПИК», Москва, Российская Федерация
• G.K. Gerasimova N.N. Blokhin Russian Cancer Research Center of RAMS, Moscow, Russian Federation ; ФГБУ «РОНЦ им. Н.Н. Блохина» РАМН, Москва, Российская Федерация

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