Immunophenotypical and cytogenetic features of tumor cells in a patient with chronic lymphocytic leukemia associated with prolonged exposure to irradiation

S.N. Kolyubaeva, I.A. Sukhina, V.Yu. Nikitin, T.V. Isakova, A.S. Polyakov, S.N. Malakhova, L.А. Myakoshina, J.N. Vinogradova, N.V. Il’yin,

DOI:

https://doi.org/10.21320/2500-2139-2013-6-3-289-293

In this article, we describe immunoрhenotypical and cytogenetic features of tumor cells in the patient with chronic lymphocytic leukemia (CLL) and prolonged exposure to irradiation in the history. Finding of 0.67% to 0.73% of metaphases with dicentric chromosomes confirmed the effect of radiation in this patient. Chromosome banding with mitogen stimulation of peripheral lymphocytes revealed 2% of cells with 47,XY, +12 karyotype, while FISH detected the greater number of cells with trisomy 12 in interphase bone marrow cells and peripheral lymphocytes. Immunophenotyping of bone marrow cells revealed the difference from “classic” CCL, i.e. simultaneous low-positive CD22 and CD79b expression as well as CD38 expression on the surface of tumor cells.

  • S.N. Kolyubaeva Military Medical Aсademy, Saint Petersburg, Russian Federation ; Военно-медицинская академия имени С.М. Кирова, Санкт-Петербург, Российская Федерация
  • I.A. Sukhina Military Medical Aсademy, Saint Petersburg, Russian Federation ; Военно-медицинская академия имени С.М. Кирова, Санкт-Петербург, Российская Федерация
  • V.Yu. Nikitin Military Medical Aсademy, Saint Petersburg, Russian Federation ; Военно-медицинская академия имени С.М. Кирова, Санкт-Петербург, Российская Федерация
  • T.V. Isakova Military Medical Aсademy, Saint Petersburg, Russian Federation ; Военно-медицинская академия имени С.М. Кирова, Санкт-Петербург, Российская Федерация
  • A.S. Polyakov Military Medical Aсademy, Saint Petersburg, Russian Federation ; Военно-медицинская академия имени С.М. Кирова, Санкт-Петербург, Российская Федерация
  • S.N. Malakhova Military Medical Aсademy, Saint Petersburg, Russian Federation ; Военно-медицинская академия имени С.М. Кирова, Санкт-Петербург, Российская Федерация
  • L.А. Myakoshina Russian Research Centre for Radiology and Surgical Technologies, RF Ministry of Health, Saint Petersburg, Russian Federation ; ФГУ «Российский научный центр радиологии и хирургических технологий» МЗ РФ, Санкт-Петербург, Российская Федерация
  • J.N. Vinogradova Russian Research Centre for Radiology and Surgical Technologies, RF Ministry of Health, Saint Petersburg, Russian Federation ; ФГУ «Российский научный центр радиологии и хирургических технологий» МЗ РФ, Санкт-Петербург, Российская Федерация
  • N.V. Il’yin Russian Research Centre for Radiology and Surgical Technologies, RF Ministry of Health, Saint Petersburg, Russian Federation ; ФГУ «Российский научный центр радиологии и хирургических технологий» МЗ РФ, Санкт-Петербург, Российская Федерация
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Keywords:

chronic lymphocytic leukemia, fluorescence in situ hybridization, trisomy 12, irradiation, radiation-specific damage

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2013-3

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Published

01.07.2013

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Vol. 6 No. 3 (2013)
LYMPHOID MALIGNANCIES

How to Cite

Kolyubaeva S.N., Sukhina I.A., Nikitin V.Y., et al. Immunophenotypical and cytogenetic features of tumor cells in a patient with chronic lymphocytic leukemia associated with prolonged exposure to irradiation. Clinical Oncohematology. Basic Research and Clinical Practice. 2013;6(3):289–293. doi:10.21320/2500-2139-2013-6-3-289-293.

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