Immunophenotypical and cytogenetic features of tumor cells in a patient with chronic lymphocytic leukemia associated with prolonged exposure to irradiation

LYMPHOID TUMORS , , , ,

S.N. Kolyubaeva1, I.A. Sukhina1, V.Yu. Nikitin1, T.V. Isakova1, A.S. Polyakov1, S.N. Malakhova1, N.V. Il’yin2, J.N. Vinogradova2, and L.А. Myakoshina2

1 Military Medical Aсademy, Saint Petersburg, Russian Federation

2 Russian Research Centre for Radiology and Surgical Technologies, RF Ministry of Health, Saint Petersburg, Russian Federation

ABSTRACT

In this article, we describe immunoрhenotypical and cytogenetic features of tumor cells in the patient with chronic lymphocytic leukemia (CLL) and prolonged exposure to irradiation in the history. Finding of 0.67% to 0.73% of metaphases with dicentric chromosomes confirmed the effect of radiation in this patient. Chromosome banding with mitogen stimulation of peripheral lymphocytes revealed 2% of cells with 47,XY, +12 karyotype, while FISH detected the greater number of cells with trisomy 12 in interphase bone marrow cells and peripheral lymphocytes. Immunophenotyping of bone marrow cells revealed the difference from “classic” CCL, i.e. simultaneous low-positive CD22 and CD79b expression as well as CD38 expression on the surface of tumor cells.

Keywords: chronic lymphocytic leukemia, fluorescence in situ hybridization, trisomy 12, irradiation, radiation-specific damage.

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REFERENCES

  1. Новик А.А. Классификация злокачественных лимфом. СПб.: ЭЛБИ, 2000. [Novik A.A. Klassifikatsiya zlokachestvennykh limfom (Classification of malignant lymphomas). SPb.: ELBI, 2000.]
  2. O’Brien S. Diagnosis and treatment of chronic lymphocytic leukemia. In: Neoplastic Diseases of the Blood, 3rd ed. Ed. by P.H. Wiernk, S. O’Brien, M.J. Keating. New York, Edinburgh, 2001: 151–9.
  3. Austen B., Skowronska A., Baker C. et al. Mutation status of the residual ATM allele is an important determinant of the cellular response to chemotherapy and survival in patients with chronic lymphocytic leukemia containing an 11q deletion. Clin. Oncol. 2007; 25: 5448–7.
  4. Bea S., Lopez-Guellermo A., Ribas M. et al. Genetics imbalances in progressed B-cell chronic lymphocytic leukemia and transformed large-cell lymphoma (Richter’s syndrome). J. Pathol. 2002; 161: 957–8.
  5. Del Giudice I., Rossi D., Chiaretti S. et al. NOTCH1 mutations in +12 chronic lymphocytic leukemia (CLL) conferan unfavorable prognosis, induce a distinctive transcriptional profiling and refine the intermediate prognosis of +12 CLL. Haematologica 2012; 97(3): 437–41.
  6. Rossi D., Rasi S., Fabbri G. et al. Mutations of NOTCH1 are an independent predictor of survival in chronic lymphocytic leukemia. Blood 2012; 119(2): 521–6.
  7. Chumak V.V., Romanenko A.Y, Voillegue P.G. et al. The UkrainianAmerican study of leukemia and related disorders among Chernobyl cleanup workers from Ukraine: II. Estimation of bone marrow doses. Rad. Res. 2008; 170(6): 691–7.
  8. Romanenko A.Y., Finch S.C., Hatch M. et al. The Ukrainian-American study of leukemia and related desorders among Chernobyl cleanup workers from Ukraine: III. Radiation risk. Rad. Res. 2008; 170: 711–20.
  9. Krestinina L.Y., Preston D.L. Ostroumova E.V. et al. Protracted radiation exposure and cancer mortality in the Techa river cohort. Res. 2005; 164: 602–11.
  10. Zent C.S., Kyasa M.J., Evans R., Schichman S.A. Chronic lymphocytic leukemia incidence is substantially higher than estimated from tumor registry date. Cancer 2001; 92: 1325–30.
  11. Богданов А.Н., Саржевский В.О., Колюбаева С.Н. и др. Случай острого лимфобластного лейкоза с t(9;22) и несколькими дополнитель- ными перестройками. Гематол. и трансфузиол. 2008; 53(30): 35–8. [Bogdanov A.N., Sarzhevskiy V.O., Kolyubayeva S.N. i dr. Sluchay ostrogo limfoblastnogo leykoza s t(9;22) i neskolkimi dopolnitelnymi perestroykami (Case of acute lymphoblastic leukemia with t(9;22) and several additional rearrangements). i transfuziol. 2008; 53(30): 35–8.]
  12. Аn International System for human Cytogenetic Nomenclature. Recommendations of the International Standing Committee on the Human Cytogenetic Nomenclature. by L. Shaffer, N. Tommerup. Basel: S. Karger, 2009.
  13. Zucchetto A. Surface-antigen expression profiling (SEP) in B-cell chronic lymphocytic leukemia (B-CLL): Identification of markers with prognostic relevance. Immunol. Meth. 2005; 305: 20–32.
  14. Escudier S.M., Pereira-Leahy J.M., Drach J.M. et al. Fluorescent in situ hybridization and cytogenetic studies of trisomy 12 in chronic lymphocytic leukemia. Blood 1993; 81: 2702–7.
  15. Schlette E. CD79b expression in chronic lymphocytic leukemia. Association with trisomy 12 and atypical immunophenotype E. Arch. Lab. Med. 2003; 123: 561–6.