Factors determining in vitro survival of human multiple myeloma cells

EXPERIMENTAL STUDIES , , ,

S.S. Shushanov1, T.A. Kravtsova1, and Yu.B. Cherhykh2

1 N.N. Blokhin Russian Cancer Research Center, RAMS, Moscow, Russian Federation

2 M.F. Vladimirskiy Moscow Regional Research-and-Clinical Institute, Moscow, Russian Federation

ABSTRACT

In this work, we investigated the survival of the human MM cell lines, i.e. RPMI1640, RPMI8226, and IM9, depending on the degree of their differentiation and impact of exogenous IGF-1. The study showed that the most and least sensitive to growth factors are IM9 cells with CD138+, CD38–, CD45+, CD56–, or CD19+ immunophenotype and RPMI1640 and RPMI8226 cells with (CD138+, CD38+, CD45–, CD56+/–, or CD19– immunophenotype, respectively The gene expression studies revealed that the level of IGF-1R and IRA mRNA expression was significantly lower in the IM9 cells than in the RPMI1640 or RPMI8226 cells. Also, we established that exogenous IGF-1 could variously influence the survival and growth of MM cells. IGF-1 alone exerts no effect on the myeloma cell viability, but, in combination with serum growth factors, it increases the parameter.

Keywords: multiple myeloma (MM), insulin-like growth factor 1 (IGF-1), cell survival, mRNA expression.

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